He utilized a light-activated.
‘But we are well located to forge ahead’. So how did Belfield develop such an ‘beyond your box’ approach? His various other nonmedical related research was the motivation. Belfield has developed a three-dimensional, optical data-storage system, that involves the use of acid generators. About six years ago he wondered if his strategy could possess applications in medical therapy. ‘It required about five years to obtain someone in my own analysis group interested to take on the unorthodox project,’ Belfield said.The effect of nonmutant DYRK1B and the R102C variant on Wnt signaling was examined in a well-established dual luciferase reporter assay,35,36 with and without Wnt3a stimulation. Wnt signaling activity was lowest in cells expressing the R102C variant, followed by cells expressing the nonmutant type, and was highest in cells transfected with empty vector . Correspondingly, cells expressing the R102C variant could actually transform into mature adipocytes without needing the addition of the adipogenic moderate . Effect of R102C Variant on Glucose-6-Phosphatase Another function of DYRK1B is the induction of the key gluconeogenic enzyme glucose-6-phosphatase.37 Increased expression38,39 and activity40 of glucose-6-phosphatase are associated with elevated fasting sugar levels in patients with type 2 diabetes.41 In a luciferase reporter assay, DYRK1B R102C significantly potentiated the induction of glucose-6-phosphatase .